By Bhushan, Vikas

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J. 35, 952 (1941). 20 A. T. Fuller, Lancet I, 760 (1942). 21 W. V. LaRosa, Proc. Soc. Exptl. Biol. Med. 53, 98 (1943). 22 M. Lederer, Australian J. Sei. 11, 208 (1949). 23 E. Kelemen, B. Tanos, and D. Halmâgyi, Biochem. J. 47, 138 (1950). 24 J. S. Lockwood, J. Immunol. 35, 155 (1938). 26 T. C. Stamp, Lancet 237, 10 (1939). 26 C. M. MacLeod, / . Exptl. Med. 72, 217 (1940). 27 J. S. Lockwood and H. M. Lynch, / . Am. Med. Assoc. 114, 935 (1940). 28 H. N. Green, Brit. J. Exptl. Pathol. 21, 38 (1940).

It may be pointed out that others have reported that PABA has a retarding effect in chronic myelogenous leukemia (see separate section) which is just the reverse of its reported effect in antagonizing a tumor-inhibiting compound. 3. HAMSTERS 106 Cooperman et al. reported that weanling hamsters fail to grow and die when receiving purified diets containing the six B vitamins—thiamine, riboflavin, calcium pantothenate, pyridoxine, nicotinic acid, and choline plus biotin. When the diet is supplemented further with PABA and inositol, there are fewer deaths.

Agarwala and Peterson43 have described procedures for the determination of PABA with the PABA-less mutant of Neurospora crassa in which the mold is grown in liquid culture. Following growth of the organism in response to PABA or unknown material, the mycelial pads are removed from the flasks in which they grew and are dried and weighed in the conventional manner. A variety of compounds (see Table IV) related to PABA in structure, including folic acid, were found to be devoid of microbiological activity.

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